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The area is located on a peninsula formed at the confluence of Julington and Durbin creeks, and has approximately nine miles of shoreline along the two creeks. The peninsula is a long sandy ridge that grades into floodplain swamp and marsh along the creeks shores. Natural communities consist of sandhill along the higher areas near the center of the peninsula and flatwoods at the lower elevations. The preserve contains extensive floodplain wetlands, providing water quality and floodplain protection for both creeks and the St. Johns River.